Bacteriological Profile and Antimicrobial Resistance of Bacteria Isolated from Pus/Wound Swabs of Diabetic Foot Patients Treated at a Tertiary Care Hospital in Bengaluru

Original Article

  • Soundarya Yamakanamardi Department of General Surgery
  • V H Ashwath Venkataramana Department of General Surgery
  • Thammegowda Kemparaj Department of General Surgery
Keywords: Antibiotic resistance, Culture and sensitivity, Diabetic foot, Wound

Abstract

Objectives of the Study: The objectives of the study were to study the pattern of bacteriological prevalence from sample isolated
by swab/pus from diabetic foot ulcerated wounds and to study the antibiotic resistance of the prevailing organisms.
Materials and Methods: Prospective study, between July 2020 and November 2020, outpatient and inpatient admitted to Bowring
and Lady Curzon and Hospitals, Bengaluru. Sample collection done using commercially available sterile cotton swabs. Gram
staining was done, followed by inoculation on the respective culture plates. Results of gram staining were typically obtained within
2 days and the culture and sensitivity reports were obtained within 5 days.
Results: Seventy-two patients, 42 were males and 30 were females. Age varied between 40 and 77 years, peak – 70 years.
Twenty-two patients suffered from peripheral arterial diseases. Thirty-two patients (44%) had peripheral neuropathy. Most common
organism isolated by culture was Escherichia coli, followed by Staphylococcus aureus. Majority of the S. aureus were sensitive to
methicillin 2 out the 72 harbored Methicillin-resistant S. aureus. Four patients suffered from Klebsiella Pneumonia, and one patient
revealed Citrobacter freundii. Almost all were uniformly resistant to penicillin, followed by tetracycline, ampicillin, and ceftriaxone.
Resistance to higher antibiotics such as colistin, piperacillin, and meropenem was also present.
Conclusion: The study determines the regional bacterial prevalence and its antibiotic resistance profile, in patients suffering
from diabetic foot. The increase in third-generation cephalosporin resistance is an alarming concern. Strict protocols need to be
developed at region levels with respect to antibiotic usage.

Author Biographies

Soundarya Yamakanamardi, Department of General Surgery

Bangalore Medical College and Research Institute, Bengaluru, Karnataka, India

V H Ashwath Venkataramana, Department of General Surgery

Bangalore Medical College and Research Institute, Bengaluru, Karnataka, India

Thammegowda Kemparaj, Department of General Surgery

Bangalore Medical College and Research Institute, Bengaluru, Karnataka, India

Published
2021-01-27
Section
Articles